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Effects of Lactobacillus paracasei subsp. paracasei NTU 101 on the cell proportions of ( A ) dead splenocytes, ( B ) CD4+/IFN-γ+, ( C ) CD4+/IL-4+, ( D ) CD4+/IL-17+, and ( E ) <t>CD4+/CD25+/FOXP3+</t> in splenocytes from EC-sensitized mice. Each value is expressed as mean ± SD ( n = 8). Statistical comparison of differences between each group: * denotes p -value less than 0.05 ( p < 0.05); ** denotes p -value less than 0.01 ( p < 0.01); *** denotes p -value less than 0.001 ( p < 0.001). The NOR group (control) received saline and a standard diet. The OVA group underwent sensitization and skin irritation with ovalbumin (OVA) on a normal diet. The CM group had the same OVA treatment plus prednisolone. The NTU 101 group received OVA treatment and NTU 101 at 1 × 10 11 CFU/day. The LGG group underwent OVA treatment and received L. rhamnosus GG ATCC 53103 at 1 × 10 11 CFU/day.
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Effects of Lactobacillus paracasei subsp. paracasei NTU 101 on the cell proportions of ( A ) dead splenocytes, ( B ) CD4+/IFN-γ+, ( C ) CD4+/IL-4+, ( D ) CD4+/IL-17+, and ( E ) <t>CD4+/CD25+/FOXP3+</t> in splenocytes from EC-sensitized mice. Each value is expressed as mean ± SD ( n = 8). Statistical comparison of differences between each group: * denotes p -value less than 0.05 ( p < 0.05); ** denotes p -value less than 0.01 ( p < 0.01); *** denotes p -value less than 0.001 ( p < 0.001). The NOR group (control) received saline and a standard diet. The OVA group underwent sensitization and skin irritation with ovalbumin (OVA) on a normal diet. The CM group had the same OVA treatment plus prednisolone. The NTU 101 group received OVA treatment and NTU 101 at 1 × 10 11 CFU/day. The LGG group underwent OVA treatment and received L. rhamnosus GG ATCC 53103 at 1 × 10 11 CFU/day.
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Effects of Lactobacillus paracasei subsp. paracasei NTU 101 on CD4 + /IFN-γ + ( A ), CD4 + /IL-4 + ( B ), CD4 + /IL-17 + ( C ), and CD4 + <t>/CD25</t> + /FOXP3 + ( D ) in splenocytes from EC-sensitized mice. Each value was expressed as the mean ± SD ( n = 8). Statistical comparison of differences between each group and the OVA group (*: p < 0.05; **: p < 0.01).
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Effects of Lactobacillus paracasei subsp. paracasei NTU 101 on CD4 + /IFN-γ + ( A ), CD4 + /IL-4 + ( B ), CD4 + /IL-17 + ( C ), and CD4 + <t>/CD25</t> + /FOXP3 + ( D ) in splenocytes from EC-sensitized mice. Each value was expressed as the mean ± SD ( n = 8). Statistical comparison of differences between each group and the OVA group (*: p < 0.05; **: p < 0.01).
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Effects of Lactobacillus paracasei subsp. paracasei NTU 101 on CD4 + /IFN-γ + ( A ), CD4 + /IL-4 + ( B ), CD4 + /IL-17 + ( C ), and CD4 + <t>/CD25</t> + /FOXP3 + ( D ) in splenocytes from EC-sensitized mice. Each value was expressed as the mean ± SD ( n = 8). Statistical comparison of differences between each group and the OVA group (*: p < 0.05; **: p < 0.01).
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Effects of Lactobacillus paracasei subsp. paracasei NTU 101 on CD4 + /IFN-γ + ( A ), CD4 + /IL-4 + ( B ), CD4 + /IL-17 + ( C ), and CD4 + <t>/CD25</t> + /FOXP3 + ( D ) in splenocytes from EC-sensitized mice. Each value was expressed as the mean ± SD ( n = 8). Statistical comparison of differences between each group and the OVA group (*: p < 0.05; **: p < 0.01).
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Effects of Lactobacillus paracasei subsp. paracasei NTU 101 on the cell proportions of ( A ) dead splenocytes, ( B ) CD4+/IFN-γ+, ( C ) CD4+/IL-4+, ( D ) CD4+/IL-17+, and ( E ) CD4+/CD25+/FOXP3+ in splenocytes from EC-sensitized mice. Each value is expressed as mean ± SD ( n = 8). Statistical comparison of differences between each group: * denotes p -value less than 0.05 ( p < 0.05); ** denotes p -value less than 0.01 ( p < 0.01); *** denotes p -value less than 0.001 ( p < 0.001). The NOR group (control) received saline and a standard diet. The OVA group underwent sensitization and skin irritation with ovalbumin (OVA) on a normal diet. The CM group had the same OVA treatment plus prednisolone. The NTU 101 group received OVA treatment and NTU 101 at 1 × 10 11 CFU/day. The LGG group underwent OVA treatment and received L. rhamnosus GG ATCC 53103 at 1 × 10 11 CFU/day.

Journal: Current Issues in Molecular Biology

Article Title: The Enhancement of Regulatory T Cell Maturation and Th1/Th2 Balance through FOXP3 Expression by Lactobacillus paracasei in an Ovalbumin-Induced Allergic Skin Animal Model

doi: 10.3390/cimb46100636

Figure Lengend Snippet: Effects of Lactobacillus paracasei subsp. paracasei NTU 101 on the cell proportions of ( A ) dead splenocytes, ( B ) CD4+/IFN-γ+, ( C ) CD4+/IL-4+, ( D ) CD4+/IL-17+, and ( E ) CD4+/CD25+/FOXP3+ in splenocytes from EC-sensitized mice. Each value is expressed as mean ± SD ( n = 8). Statistical comparison of differences between each group: * denotes p -value less than 0.05 ( p < 0.05); ** denotes p -value less than 0.01 ( p < 0.01); *** denotes p -value less than 0.001 ( p < 0.001). The NOR group (control) received saline and a standard diet. The OVA group underwent sensitization and skin irritation with ovalbumin (OVA) on a normal diet. The CM group had the same OVA treatment plus prednisolone. The NTU 101 group received OVA treatment and NTU 101 at 1 × 10 11 CFU/day. The LGG group underwent OVA treatment and received L. rhamnosus GG ATCC 53103 at 1 × 10 11 CFU/day.

Article Snippet: Cell staining was performed using a panel of monoclonal antibodies: FITC-labeled anti-mouse CD4 (clone: RM4-5, eBioscience, San Diego, CA, USA); PE-labeled rat anti-mouse CD25 (clone: PC61.5, eBioscience); PerCP-Cy™ 5.5-labeled rat anti-mouse IFN-γ (clone: XMG1.2, BD, NJ, USA); PerCP-Cy™-labeled rat anti-mouse IL-4 (clone: 11B11, BioLegend, San Diego, CA, USA); PerCP-Cy™ 5.5-labeled rat anti-mouse FOXP3 (clone: FJK-16s, Invitrogen, Carlsbad, CA, USA); and PerCP-Cy™ 5.5-labeled rat anti-mouse/rat IL-17A (clone: eBio17B7, eBioscience).

Techniques: Comparison, Control, Saline

Effects of Lactobacillus paracasei subsp. paracasei NTU 101 on CD4 + /IFN-γ + ( A ), CD4 + /IL-4 + ( B ), CD4 + /IL-17 + ( C ), and CD4 + /CD25 + /FOXP3 + ( D ) in splenocytes from EC-sensitized mice. Each value was expressed as the mean ± SD ( n = 8). Statistical comparison of differences between each group and the OVA group (*: p < 0.05; **: p < 0.01).

Journal: Current Issues in Molecular Biology

Article Title: The Beneficial Role of Lactobacillus paracasei subsp. paracasei NTU 101 in the Prevention of Atopic Dermatitis

doi: 10.3390/cimb46030143

Figure Lengend Snippet: Effects of Lactobacillus paracasei subsp. paracasei NTU 101 on CD4 + /IFN-γ + ( A ), CD4 + /IL-4 + ( B ), CD4 + /IL-17 + ( C ), and CD4 + /CD25 + /FOXP3 + ( D ) in splenocytes from EC-sensitized mice. Each value was expressed as the mean ± SD ( n = 8). Statistical comparison of differences between each group and the OVA group (*: p < 0.05; **: p < 0.01).

Article Snippet: The BD Cytofix/CytopermTM Kit (Cat. No. 554714, BD) was used for fixing and cell membrane perforation, and paired staining was performed using the following monoclonal antibodies: FITC anti-mouse CD4 monoclonal antibody (clone: RM4-5, eBioscience, San Diego, CA, USA); PE rat anti-mouse CD25 monoclonal antibody (clone: PC61.5, eBioscience); PerCP-CyTM 5.5 rat anti-mouse IFN-γ monoclonal antibody (clone: XMG1.2, BD); PerCP-CyTM rat anti-mouse IL-4 monoclonal antibody (clone: 11B11, BioLegend, San Diego, CA, USA); PerCP-CyTM 5.5 rat anti-mouse FOXP3 monoclonal antibody (clone: FJK-16s, Invitrogen, Carlsbad, CA, USA); PerCP-CyTM 5.5 rat anti-mouse/rat IL-17A monoclonal antibody (clone: eBio17B7, eBioscience).

Techniques: Comparison